Spectroscopic revelation

This video by Minu Mathen from India seeks to elucidate the molecular changes in anterior lens capsule after staining with different concentrations of Trypan blue dye at various time intervals. Anterior lens capsules were collected after capsulorhexis from 20 patients. The capsules were exposed to various time intervals and dilutions of dye. Raman laser spectrometry was used to get spectra from unstained and stained capsules, and computerised data plotting done.