DIVERSITY AND COMPOSITION OF OCULAR MICROBIOTA IN CONTACT LENS WEARERS: EFFICACY OF LIPOSOMAL OZONATED OIL
Published 2025 - 29th ESCRS Winter Meeting
Reference: PO068 | Type: Presented Poster & Poster | DOI: 10.82333/1c2z-pk80
Authors: María Carmen Sánchez-González* 1 , Concepción De-Hita-Cantalejo 1 , Estanislao Gutiérrez-Sánchez 2 , Raúl Capote-Puente 1 , José-María Sánchez-González 1
1Physics of Condensed Matter,University of Seville,Seville,Spain, 2Surgery,University of Seville,Seville,Spain
Purpose
To characterize the ocular surface microbiota in regular contact lens wearers with dry eyes and assess the effectiveness of reducing bacterial load using a liposomal ozonated oil solution.
Setting
The research followed the tenets of the Declaration of Helsinki and was approved by the Andalusian Biomedical Research Ethics Committee (SICEIA-2024-000925) and conducted at the Optics and Optometry facilities of the Pharmacy School, University of Seville (Seville, Spain).
Methods
This prospective, longitudinal, controlled study randomized subjects into two groups. Group A (45 subjects) received hydroxypropylmethylcellulose (HPMC, Artific®), while Group B (41 subjects) received ozonated sunflower seed oil with soybean phospholipids (OSSO, Ozonest®). Microbial communities were analyzed via DNA metabarcoding of the 16S rRNA gene, and statistical analyses (alpha and beta diversity) were performed in R.
Results
Both groups predominantly harbored Staphylococcus caprae, Streptococcus oralis, and Corynebacterium spp., with OSSO and HPMC users showing distinct bacterial profiles. Alpha diversity showed no significant differences, but beta diversity revealed differences in bacterial composition between the groups.
Conclusion
The results seem to indicate that the use of ozonized oil reduces the bacterial load compared to the solution used as a control.