UP-REGULATION OF INNATE IMMUNE RELATED GENES IN MONONUCLEAR CELLS OF STEVENS-JOHNSON SYNDROME WITH SEVERE OCULAR COMPLICATIONS
Published 2025 - 29th ESCRS Winter Meeting
Reference: PO019 | Type: Presented Poster & Poster | DOI: 10.82333/wp5z-4k12
Authors: Mayumi Ueta* 1 , Hiromi Nishigaki 1 , Hokoru Yoshioka 1 , Shigeru Kinoshita 1 , Chie Sotozono 1
1Ophthalmology,Kyoto Prefectural University of Medicine,Kyoto,Japan
Purpose
Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are severe mucocutaneous disorders characterized by extensive tissue necrosis and are often accompanied by severe ocular complications (SOC). Because innate immune-related genes were positively regulated by hsa-let-7a-5p and negatively regulated by hsa-miR-628-3p which were upregulated in the plasma of SJS/TEN with SOC, we have suspected innate immune-related genes might contribute to the development of ocular surface inflammation. This study aimed to explore the expression profiles of innate immune-related genes mRNAs in the mononuclear cells of patients with SJS/TEN with SOC.
Setting
Case Control Study
Methods
We conducted RT-qPCR on mononuclear cells from 12 SJS/TEN patients and 9 controls to assess the expression of the innate immune-related genes such as IFI44L, TNFSF10, AIM2, RSAD2, CXCL10, TRIM22, IFI27, and IFIT2, in addition of TLR3, RIG-I, and MDA5.
Results
MDA5, IFI44L, RSAD2, CXCL10, and IFIT2 were significantly up-regulated in the mononuclear cells from these patients, indicating a systemic modulation of immune response genes.
Conclusion
Our findings might underscore the critical role these genes play in mediating the immune responses characteristic of SJS/TEN with SOC.